human monoclonal antibodies against il 1rii  (R&D Systems)

Journal: Cardiovascular Drugs and Therapy

Article Title: Colchicine Attenuates Microvascular Obstruction after Myocardial Ischemia-Reperfusion Injury by Inhibiting the Proliferation of Neutrophil in Bone Marrow

doi: 10.1007/s10557-023-07528-y

Figure Lengend Snippet: Colchicine inhibited neutrophil proliferation and Il-1β receptor expression in the BM of mice after myocardial I/R. ( A ) Representative flow cytometry plots and ( B ) statistical analysis of CD45 + Ly6G + neutrophils (as assessed by EdU incorporation) in the BM of mice at 12 h after myocardial I/R (n = 4 each group). ( C ) Representative immunohistochemistry images of IL-1RI, IL-1RII, and IL-1Ra in BM of mice at 12 h after myocardial I/R (scale bar = 20 μm). ( E–F ) Quantification of IL-1RI, IL-1RII, and IL-1Ra positive area in BM of mice at 12 h after I/R (n = 3 each group). Data are shown as mean ± SD. ns, not significant, * P < 0.05, *** P < 0.005, **** P < 0.001. BM, bone marrow. Col, colchicine. ABR-215757, S100A8/A9 inhibitor

Article Snippet: Paraffin sections were deparaffinized and rehydrated, then deposited and stored at 4 °C overnight before being incubated with primary antibodies IL-1RI (1:50, sc-393998, Santa Cruz, USA), IL-1RII (1:50, sc-376247, Inc), IL-1RII (1:50, sc-376247, Santa Cruz, USA), and IL-1Ra (1:50, sc-374084, Santa Cruz, USA).

Techniques: Expressing, Flow Cytometry, Immunohistochemistry